肠腺无细胞滤波对受照射小鼠肠腺再生的影响

EFFECTS OF THE CELL-FREE FILTRATES OF THE CRYPT CELL SUSPENSION ON THE CRYPT REGENERATION IN THE IRRADIATED MICE

用刮取法制备小鼠肠腺细胞悬液,分别经冻融和超声处理,并抽滤,获得肠腺无细胞滤液。BALB/c小鼠经15Gyγ线全身照射后,注入肠腺无细胞滤液或肠腺细胞悬液。72h后照射对照空肠段除有极少数残存肠腺外,已无肠腺再生;而照射注入组注入肠段有较多的新肠腺形成,肠腺内细胞生长良好,并可见未分化细胞。在9.4 Gyγ线腹部照射实验中观察到,照射并注入无细胞滤液后14d时,新肠腺仍持续存在。本实验结果提示,在肠腺无细胞滤液中,存在着某种肠腺修复因子,它能促进受照射小鼠肠腺的修复再生。

Two cell-free filtrates were obtained by filtering the fresh crypt cellsuspensions which were pretreated with freeze-thawing and ultrasonic disintegrationrespectively through the G_6. funnel. 0.4 ml of each filtrate or the fresh crypt cellsuspension was administered separately into the lumen of a 3 em jejunal segment inBALB/c mouse 1-3 hours following 15 Gy γ-ray total-body irradiation. Seventy-twohours later,no regenerating crypt could be found and the villi become remarkablyshorter in the irradiated control, while in the jejunal segments receiving both filtra-tes or the fresh crypt cell suspensions, numerous newly-formed crypts were visiblewith the villi apparently normal; meanwhile, the columnar, goblet, argentaffin, Panethcells as well as undifferentiated cells could. be identified in the crypt region under ele-ctron microscope. The new crypts were found to exist persistently in 14 days' experi-mental period after 9. 4 Gy γ-ray abdominal irradiation and filtrates administration.The above findings suggest the presence of a crypt repair factor in cell-free filtratespyepared from the intestinal crypt cell suspension of normal mice.