高强脉冲电场对食品中大肠埃希氏菌基因水平传递的影响

Effects of pulsed electric fields on the gene horizontal transfer of Escherichia coli

目的:探讨高强脉冲电场对大肠埃希氏菌的基因获得及水平传递中的作用。方法:采用接合转移试验,研究了PEF强电场作用对大肠埃希氏菌基因水平传递的影响,并以未经处理的标准菌株为对照,通过PCR方法进行基因检测分析。结果:所有经PEF处理的菌株均由正常的接合转移率42.4%～68.5%上升到100%。PCR检测结果显示,所得接合子中int基因的扩增产物大小仍为923bp,与正常未处理int基因大小一致。结论:PEF处理使得细菌接合转移率明显提高,PEF处理对传递到接合子中的基因未能产生影响。

Objective： The effect of pulsed electric fields（PEF） on the gene horizontal transfer of E coli was studied in this paper. Methods： Conjugation method was used and PCR method established to analysis the gene difference. Result： The conjugation rate of the strains treaded with PEF was raised from normal 42.4%-68.5% to 100%. PCR result was indicated that the strains treaded with PEF present amplicons about 923bp as normal. Conclusion： It was shown that there exists the influence of pulsed electric fields on the gene horizontal transfer of E coli.