低聚壳聚糖制备及黏均分子量的测定

Preparation and measurement of molecular weight of low-molecular weight chitosan

研究了壳聚糖的降解和黏均分子量的测定,确定了壳聚糖降解的适宜条件。亚硝酸钠降解壳聚糖的最佳条件是10% NaNO2用量0.6mL、温度35℃、时间35min、醋酸浓度3%;双氧水降解的最佳条件是H2O2浓度6%、温度70℃、时间6h、醋酸浓度4%;中温α-淀粉酶的最佳工艺条件为温度40℃、时间90min、酶用量1200U/g。

This paper introduces degradation of chitosan and maesurement of molecular weight, and get the appropriate condition.The best conditions of degradation of chitosan by nitrite sodium： 10% NaNO2 is 0.6mL, creaction temperature is 35℃, reaction time is 35min and CH3COOH concentration is 3%; The best conditions of degradation of chitosan by hydrogen peroxide： H2O2 concentration： 6%, creaction temperature is 70℃, reaction time is 6h and CH3COOH concentration is 4%; The best conditins of degradation of chitosan by common α-amylase： creaction temperature is 40℃, reaction time is 90min, dosage of enzyme 1200U/g.