摘要
本研究工作采用放射免疫分析、荧光检测及高效液相色谱分析等技术,测定了大鼠经10 GyX射线头部照射后,脑内单胺类化合物与某些神经肽含量的变化。结果表明:头部照射后第2d,下丘脑中irL-Enk和垂体irM-Enk明显减少;而下丘脑NE、E和DA,间脑中5-HIAA及其与5-HT的比值明显增高。其它脑中的单胺类化合物变化不显著。提示下丘脑具有较高的辐射敏感性,并且下丘脑所发生的上述变化将对神经与内分泌系统的机能活动产生重要影响。
In the present study radioimmunoassay, fluorimetry and high perfo-rmance liquid chromatography were used to determine neuropeptides and monoamineneurotransmitters in the rat brain after head irradiation with 10 Gy X-rays. The resu-lts showed that the immunoreactive (ir) L-Enk in the hypothalamus and irM-Enk inthe pituitary decreased markedly,while the contents of NE, E and DA in the hypot-halamus, 5-HIAA in the diecephalon and the 5-HIAA to 5-HT ratio increased signi-ficantly. The monoamines in the other cerebral area showed no significant changes.These results suggest that the hypothalamus is comparatively radiosensitive and neuro-chemical changes in it may have profound influence on the functions of the nervousand endocrine system.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1990年第4期235-239,共5页
Journal of Radiation Research and Radiation Processing
关键词
头部
电离
辐射
单胺类
介质
Head irradiation
Monoamine neurotransmitters
Neuropeptides
Hypothalamus
