用荧光探针法观察离体与整体照射对大鼠红细胞膜流动性的影响

EFFECT OF GAMMA IRRADIATION ON ERYTHROCYTE MEMBRANE IN RATS BY FLUORESCENT PROBES IN VITRO AND IN VIVO

ANS(1-苯胺-8-萘磺酸)和DPH(1,6-二苯基-1,3,5,-己三烯)作为探针,用于检测γ射线对大鼠红细胞膜的作用。离体照射大鼠红细胞膜,随着剂量的增加(10—40 Gy),ANS结合红细胞膜的荧光强度降低。用DPH作为荧光探针,在整体实验中也得到了同样的结果,全身照射8.5 Gy之后的第1、3和5d,DPH结合大鼠红细胞膜的荧光强度下降,荧光偏振度增加。这些结果反映了照射使膜的流动性降低,可能是由于γ射线离体与整体照射诱导的膜不饱和脂肪酸的脂质过氧化及膜蛋白构象改变引起的。

Fluorescent dyes, 1-anilino-naphthalene sulfonate (ANS) and 1,6-diphenyl-1, 3,5-hexatriene (DPH), were used as probes to examine the effect of γ-irradiation on the rat erythrocyte membrane. After irradiation of rat erythrocyte membrane in vitro, the fluorescence intensity ofmembrane-associated ANS decreased with the increase of irradiation dose (10-40 Gy). Similar results were also observed in the experiment of irradiation in vivo asshowed by the decrease of fluorescence intensity of DPH probe in the erythrocytemembrane as well as the increase of its polarization on the 1 st, 3 rd, 5 th dayfollowing whole body irradiation with 8 .5 Gy. Those results reflected the decrease of membrane fluidity which was suggested to beresulted from lipid peroxidation of polyunsaturated fatty acid in membrane induced byγ-irradiation in vitro and in vivo.