白术多糖对仔猪血淋巴细胞转化及信号转导相关分子的影响

Effect of PAM on Proliferation of Lymphocytes and on Signaling Related Molecules in Peripheral Blood of Piglet

本试验研究了白术多糖对仔猪血淋巴细胞转化率及细胞内信息分子一氧化氮(NO)、环腺苷酸(cAMP)、环鸟苷酸(cGMP)及干扰素γ(IFN-γ)的影响。分离制备仔猪血淋巴细胞悬液,MTT法检测细胞增殖;酶联免疫法测定培养上清液IFN-γ的浓度;放射免疫法测定cAMP、cGMP的浓度;利用硝酸还原法测定NO,发现白术多糖能显著促进仔猪血淋巴细胞转化率I、FN-γ、细胞内NO浓度及cAMP浓度。以上结果提示,白术多糖能显著提高血淋巴细胞免疫功能,其作用是通过改变细胞内信息传导实现的。

The experiment was conducted to study the effect of polysaccharide from atractylodis macrocephalae （PAM） on proliferation of lymphocytes and signaling related molecules like NO, cAMP and cGMP in peripheral blood of piglet. Effects of PAM on proliferation of peripheral blood lymphocytes of piglet were tested by MTT assay. The production of IFN-γ in supernatant were determined by ELISA ; the content of cAMP and cGMP were tested by RIA. The concentration of NO was tested by deoxidization assay. The results showed that PAM insignificantly increased proliferation of lymphocytes and the contents of NO, cAMP and IFN-γ in similar dose dependent manner. In conclusion, PAM might perform its immunoregulation functions via signal transduction system in immunocytes by activating immunocytes to release NO and cAMP/cGMP and raise IFN-γ.