摘要
从北京油鸡盲肠黏膜中分离得到1株戊糖乳酸杆菌M8,在通气和密闭培养条件下对生长OD600nm值和pH值进行了测定。结果显示:M8在通气培养条件下OD600nm最高值为1.011,pH值最低为4.40,而密闭培养条件下OD600nm达2.049,pH降至3.42,确定密闭培养为最佳培养条件。密闭培养条件下,在培养基中添加不同比例的菊粉(1%,2%,3%,4%),探讨菊粉对菌株生长情况的影响,结果发现:当菊粉添加量为3%时效果最佳,其OD600nm最高达2.937,pH最低降至3.40。
In the experiment, we isolated 1 strain lactobacilli which was defined as Lactobacillus Pentosus （M8） from blind intestine of healthy Beijing You fowl. We got its growth curves and best culture condition in breezy and sealed bottles. The result showed： OD600nm reached to 1.011 and pH reached to 4.40 in breezy bottles. On the other hands, in the sealed bottles, OD600nm reached to 2. 049 and pH reached to 3.42. Therefore, its optimum culture condition was in sealed bottles. We also got the growth OD600nm and pH in sealed bottles with different levels of inulin. The result was that the best peroent of inulin was 3 %, and the OD600nm reached to 2. 937 and pH reached to 3.40 .
出处
《华北农学报》
CSCD
北大核心
2007年第2期171-174,共4页
Acta Agriculturae Boreali-Sinica
关键词
乳酸杆菌
培养条件
培养基
菊粉
Lactobacillus
Culture condition
Culture medium
Inulin
