摘要
Objective： To investigate the effect of moxibustion on telomerase activity and genes expression in tissues of senescent rats. Methods： Subacute aging rats model were established by injection with D-gal solution. Points Shenshu （BL 23） were treated with moxibustion in treatment group, contrasting with a model group and a normal group. Enzyme-Linked Immunosorbent Assays（ELISA） was used for the level of telomerase activity in liver tissues, and In Situ Hybridization（ISH） was used for the condition of expression of telomerase genes in liver tissues. Results： The level of telomerase activity in the aging model group was obviously lower than that in normal control group （P〈0.01）, the level in moxibustion group was obviously higher than that in model group（P〈0.05）. In comparison with normal rats, the positive-expressed areas and photodensity of telomerase genes in aging model group were all significantly lower （P〈0.01, P〈0.01）, and the positive-expressed areas in moxibustion group was significantly higher than that in model group （P〈0.01）. Conclusions： Moxibustion could regulate telomerase activity of senile rats, hence delaying aging.
目的：研究艾灸对衰老大鼠组织端粒酶活性及基因表达的影响。方法：以注射D．半乳糖溶液法制备亚急性衰老大鼠模型，采用艾灸“肾俞”穴进行治疗，并与模型组和正常组进行对照，分别以ELISA法和原位杂交观察各组大鼠肝脏组织端粒酶活性及其基因的表达的变化。结果：衰老大鼠端粒酶活性较正常组显著降低（P〈0．01），艾灸组与模型组比较明显升高（P〈0．05）。与正常组相比，衰老大鼠端粒酶基因阳性表达面积和阳性表达光密度均显著降低（P〈0．01），艾灸组阳性表达面积较模型组显著增高（P〈0．01）。结论：艾灸可通过调节衰老大鼠的端粒酶活性从而达到延缓衰老的目的。
