脱水螺旋藻细胞复水后在不同光强下的谱学性质

The Spectra of Water-Reabsorbing Spirulina Maxima at Different Light Intensity

将冷冻干燥处理后的极大螺旋藻干粉(脱水细胞)悬浮于NaCl等渗盐水中并添加葡萄糖和尿素,分别置于暗室、自然光照环境和培养箱中,在2,14,26h后分别测定其吸收光谱、荧光发射和激发光谱的变化。结果表明:在相同时间下,藻细胞复水后的光谱特征峰随光强的不同呈现有规律的下降,暗室下的复水藻细胞特征峰强度最高,而放置在光照培养箱的则最低。说明在水分胁迫和光抑制的双重作用下,经过冷冻脱水的藻细胞复水后无法恢复其正常的光合作用。

The freeze-dried cells of Spirulina maxima were resuspended in NaCl isotonic solution under the condition of darkness, natural light and culture illumination respectively. Their spectra were studied after the Spirulina maxima was treated for 2, 14 and 26 h respectively. The results indicated that the peak intensity decreased gradually at different light intensity but in the same time span. The peak intensity was the highest when Spirulina maxima was put in darkness, but lowest under culture illumination. The water-reabsorbing Spirulina maxima couldn＇t restore the normal photosynthesis under the dual stress of both water and light.