茶炭疽病菌毒素的致病活性及理化性质初探

Preliminary Studies on Pathogenic Activity and Physical and Chemical Characteristics of Toxins from Gloeosporium theae-sinensis

研究发现茶炭疽病菌Gloeosporium theae-sinensis Miyake产生有致病力的外毒素,引起茶树叶片坏死,形成枯斑,类似于病原菌侵染形成的症状。对该菌的培养毒素滤液进行了生物测定,以探讨最佳产毒条件和理化性质。得最佳产毒条件:pH=5.0、25℃且于Czapek-Dox培养液中连续振荡培养16d。将茶梢浸入毒素滤液而感毒,而后用于茶炭疽病菌毒素生物测定的方法比较合适。

Tea anthracnose fungi, Gloeosporium theae-sinensis, can produce pathogenic exo-toxin, which induced the lesions of tea leaves and the symptoms being similar to those resulted from G. theae-sinensis in the fields. The cultural filtrate of G. theae-sinensis was used to toxin bioassay, so as to make a study of the optimum conditions for toxin-producing and characteristics of toxins. The optimum toxin-producing conditions were as follows： pH 5.0, and 25 ℃ cultivating for 16 days in the manner of vibration with Czapek-Dox liquid medium. The method of immerging the tea shoots into toxin solution was appropriate for the bioassay of toxins.