海洋真菌液体发酵产淀粉酶的研究

Submerged Fermentation of Amylase by Marine Fungus

研究了分离自乐清湾红树林的1株海洋真菌(真菌#34)液体发酵产淀粉酶的条件．结果表明,最优培养条件为:培养时间95 h、培养温度30℃、起始pH值5．8、盐度1(即天然海水);最优培养基配方为:淀粉3 g、麸皮14 g、酵母膏6 g、KH_2PO_4 0．5 g、MgSO_4·7H_2O 0．4 g、海水1000 mL．

The paper has studied the conditions of marine fungus #34, which is separated from the mangrove in the Yueqing Bay. The marine fungus #34 produces amylases by submerged fermentation under the conditions. The results shows that the optimum conditions are as followed： fermentation time of 95h, fermentation temperature of 30℃ and initial pH of 5.8 and salinity of 1 of the media. The optimum formula of the media, which are determined to use the uniform design test, are as followed： starch 3 g, bran 14 g, yeast extract 6 g, KH2PO4 0.5 g, MgSOao7H20 0.4 g and sea water 1000 mL.