微环境调控对灯盏花无糖组培苗显微结构的影响

Effect of Micro-environment Control on Microstructure of Plantlet in Sugar-free Tissue Culture of Erigeron breviscapus

以灯盏花组培苗为材料,采用扫描电镜和光学显微镜对灯盏花无糖组培苗及有糖组培苗(CK)的叶表皮毛、气孔、蜡质层和根部的显微结构进行了比较分析。研究结果表明:灯盏花无糖组培苗根、叶的显微结构与有糖组培苗有明显区别,无糖组培微环境调控技术对提高组培苗的保水能力及适应外界环境的能力具有重要作用。

In this research, adventitious bud of Erigeron breviscapus were used as materials. And analyzed the four characteristics of the plantlet of Erigeron breviscaptts cultured under the condition of sugar-free and sugar （CK） by using scanning electron microscopy （SEM） and optical microscopy, inclu- ding the foliar trichome, stomatal, epicuticular wax, the microstructure of roots. Results showed that the microstructure of roots and leaf have the obvious difference between them, and the micro-environment control technology has the important effect to improve the capability of root to hold water and adjust to the external environment in sugar-free tissue culture.