胰岛素样生长因子1转化番茄的研究

Primary Study on Transformation of Tomato with Insulin-like Growth Factor-1

以MS为基本培养基,附加不同浓度BA与IAA激素组合,对番茄子叶和下胚轴进行离体培养,结果子叶芽诱导的效果明显好于下胚轴,最佳分化培养基为MS+BA 3.0 mg/L+IAA 0.15 mg/L。采用根癌农杆菌介导法,将胰岛素样生长因子1(Insulin-like Growth Factor-1,IGF-1)基因导入番茄中,通过多批次转化及筛选,最终获得15株抗性植株,PCR检测全部呈阳性,初步表明IGF-1基因已整合到番茄基因组中。

In this study, MS medium supplemented with different combinations of different concentrations of BA and IAA was used to culture tomato cotyledons and hypocotyls. The effective culture in vitro system of this cuhivar was established. The optimum inductive medium was MS ＋ BA 3. 0 mg/L ＋ IAA 0. 15 mg/L. Cotyledon explants of tomato seedlings were infected with Agrobacterium tumefaciens strain EHA105 harboring a plasmid vector containing IGF - 1 gene. 6 transformation experiments were carried out. 15 resistant shoots were obtained after 4 times of successive kanamycin-resistant selected culture （ MS ＋ BA 3.0mg/L ＋ IAA 0. 15 mg/L ＋Km 30 mg/L ＋ Cb 400 mg/L）. The resistant regenerated plants were tested by PCR analysis, which showed IGF - 1 gene was integrated into tomato genome.