环氧合酶-2在子痫前期患者胎盘组织中的表达

The Study of COX-2 in preeclampsia

目的研究环氧合酶-2(COX-2)在子痫前期患者胎盘组织中的表达,探讨其在子痫前期发病机制中的作用。方法采用免疫组化SP法检测50例子痫前期患者(轻度30例,重度20例)胎盘组织中COX-2的表达,20例正常孕妇为对照组。应用逆转录多聚酶链反应(RT-PCR)检测30例子痫前期患者及12例正常孕妇的胎盘组织中COX-2mRNA的表达。结果(1)COX-2主要表达于胎盘血管内皮细胞。子痫前期患者COX-2蛋白的表达均明显增高,且随病情严重程度的增加而升高。子痫前期组COX-2蛋白的阳性表达率为63.1%、显著高于对照组(COX-2阳性表达率为23%)(P<0.01)。(2)子痫前期组COX-2mRNA表达水平为0.61±0.25,对照组为0.66±0.71,两组比较,差异无显著性(t=-0.93,P>0.05)。结论COX-2参与了子痫前期胎盘螺旋小动脉动脉粥样硬化。

Objective： The purpose of the present study was to explore the expression of cyclooxyygenase- 2 （COX -2） in placentas with preeclampsia （PE）. Methods： 50 women with PE in the third trimester of pregnancy and 20 normotensive women in the third trimester of pregnancy were involved. The patients with PE were divided into 2 groups. The protein and mRNA were determined by immunohistochemistry and RT - PCR. Results ： The expression level of COX - 2 protein in preeclampsia group was significantly higher than that of control group （ P 〈 0. 05 ）, while no significant differences were noted between the mRNA levels in the preeclamptic women and those in normotensive pregnant women [ （0. 61±0. 5） vs （0. 66±0. 71 ）, P 〉0. 05]. Conclusion： Our findings suggest that the increased COX - 2 may be related to the atheroscleroesis of PE.