摘要
建立了枇杷多糖的提取纯化方法,并对提取的枇杷多糖进行了理化性质分析。枇杷多糖经40%乙醇分级得到的组分Lens5,用Sephadex G-150凝胶柱层析得到单一峰,Lens5的酸性水解液经纸层析和薄层层析分析,其单糖组成为葡萄糖和木糖,用Sephadex G-150凝胶柱层析法测得其分子量为43000Da。
In this study a method of Loquat polysaccharide isolation and purification was setup and the physical and chemical characteristics of Loquat polysaccharide was also analyzed.Product Len5 was obtained when Loquat polysaccharide was classified with 40 % ethanol, and a single apex was obtained in the Sephadex G-150 gel chromatography analysis. The monosaccharide components of the acidic solution of Lens5 were identified as glucose and xylose according to the results of paper chromatography and thin layer chromatography analysis.The molecular weight of Len5 is 43 000 Dalton according to the result of Sephadex G-150 gel chromatography analysis.
出处
《食品研究与开发》
CAS
北大核心
2007年第4期14-16,共3页
Food Research and Development
关键词
枇杷
多糖
分离纯化
理化性质
Loquat
polysaccharide
isolation and purification
physical and chemical characteristic
