错配修复基因hMLH1、hMSH2及p53在子宫内膜癌中的表达及意义

Expression of human mismatch repair gene(hMLH1/hMSH2) and p53 overexpression in endometrial carcinoma and its significance

目的探讨错配修复基因hMLH1、hMSH2及p53在子宫内膜癌组织中的表达及意义。方法运用免疫组织化学S-P法对99例子宫内膜癌中hMLH1、hMSH2及p53的表达进行检测。结果99例子宫内膜癌组织中hMLH1与hMSH2的阳性表达率分别为38.3%和60.6%,与内膜癌临床分期、组织类型及组织学分化程度无关(P>0.05);99例内膜癌组织中p53阳性表达率为42.4%,组织分化程度高、临床分期早的内膜癌组织中的p53阳性率明显低于分化程度低及临床分期晚者(P<0.01);hMSH2蛋白阳性组中p53表达率明显高于阴性组(P<0.01)。结论hMLH1及hMSH2基因的缺陷及p53的表达与子宫内膜癌的发生发展过程有关。

Objective To investigate the expression of human mismatches repair gene （hMLH1/hMSH2） and p53 in ertdometrial cancer and its significance. Methods Expression of hMLH1/hMSH2 and p53 in 99 cases with endometrial cancer was examined by S-P immunohistochemical staining. Results In 99 cases with endometrial cancer, the positive expression rate of hMLH1 and hMSH2 was 38.3% and 60.6% respectively. It wasn＇t positively correlated with FIGO grade, pathological types and histologic stage. In 99 cases with endometrial cancer, the positive expression rate of p53 in the patients was 42.4 %, the positive expression rate of p53 in high grade and early stage was significantly lower than that in low grade and late stage （P 〈 0.01）. The postitive expression rate of p53 in hMSH2 positive endometrial cancer tissue was significantly higher than that in hMSH2 negative tissue （P 〈 0.01 ）. Conclusion The abnormal expression of hMLH1/hMSH2 and p53 might be involved in the carcinogens process of endometrial cancer.