摘要
目的测定缩宫素的含量。方法采用高效液相色谱梯度洗脱法,选用Diamonsil C_(18)柱,以15.6 g/mL磷酸二氢钠溶液及乙腈-水(1:1)为流动相梯度洗脱,检测波长为220 nm。结果线性范围为0.5～100 IU/mL。平均加样回收率为96.9%。结论高效液相色谱法所用方法准确、简便。
Objective To establish a HPLC method for assaying of oxitocin. Methods The gradient elution was used with Diamonsil C18 column and UV detection at 220 nm. The mobile phase was 15.6 g/mL sodium dihydrogen phosphate buffer and acetonitrile-water（1 ： 1）. Results The oxitocin curves were linear between 0.5- 100 IU/mL（r = 1. 000 0）,and the recovery rate of the standards added was 96. 9%. Conelusion The method is accurate,simple.
出处
《中国药业》
CAS
2007年第8期31-32,共2页
China Pharmaceuticals
