金黄色葡萄球菌半乳糖醇酶基因克隆

Cloning of dulcitol enzyme gene of staphylacoccus aureus.

目的了解金黄色葡萄球菌的生物化学和代谢特征,以及其半乳糖醇酶与产生肠毒素的关系。方法利用PCR方法从金黄色葡萄球菌标准株中扩增出金黄色葡萄球菌半乳糖醇酶,克隆到pmD-18T载体上并转化到E.coliJM109菌株。结果用特异引物扩增800bp的基因片段,目的基因被克隆,经双酶切和DNA序列测定为金黄色葡萄球菌半乳糖醇酶基因。结论金黄色葡萄球菌半乳糖醇酶基因片段可以被特异性扩增和克隆。

Objective To understand the character of biochemistry and motabelism of Staphylococcus aureus , and relationship between entertoxin produet and expression of duleitol enzyme gene. Methods Duleitol enzyme gene of Staphylococcus aureus was amplified from Staphylococcus aureus genome with PCR, gene was eloned into pmD- 18T, then was transferred to E.eoli JM109. Results 800bp gene fragment was amplified by PCR from Staphylococcus aureus genome, and also was cloned plasmld pmD- 18T, farget gene was identified from transferred on to plasmid pmD - 18T by the way of DNA sequence and double enzyme eut. Conclusion Duleitol enzyme gene of Staphylococcus aureus could be amplified and cloned specially.