摘要
毛细管等电聚焦（CIEF）可分离等电点（pI）相差0．02pH单位的蛋白质。本文介绍了CIEF分离蛋白质的基本原理、方法和特点；阐述了CIEF分离蛋白质应解决的基本问题，包括消除或减小电渗的涂层方法。检测方法和条件。蛋白质区带的迁移及pI测定方法。讨论了影响CIEF分离效能的因素，如两性电解质的组成和浓度、毛细管长度、聚焦电压等。列举了应用CIEF分离具有不同pI蛋白质混合物的实例，对CIEF的应用前景进行了展望。
Capillary isoelectric focusing (CIEF) can separate proteins with pls different from each other by 0.02 pH unit. The basic principles. separation methods and characteristics of CIEF are described. Application problems including electroosmosis,detection techniques and the migration of focused zones are described also. The factors that affect the separation efficiency are discussed including the composition and concentration of ampholytes. the length of capillary and the focusing voltage. Examples of practical applications for the separation of protein mixtues are presented.
出处
《分析仪器》
CAS
1997年第2期52-56,共5页
Analytical Instrumentation
