活血丹组织培养与快速繁殖技术研究

Studies on tissue culture and rapid propagation techniques of Glechoma longituba

以活血丹为材料,应用组织培养和快速繁殖技术,对适于活血丹增殖分化的培养基、培养方式进行了系统的研究。用活血丹叶片作为外植体,在MS添加生长素2,4-D和细胞分裂素BA的培养基上成功诱导出愈伤组织,并对其继代培养条件进行研究,分析了继代培养中褐化的原因。在MS添加NAA和BA的培养基中,活血丹的茎尖和带腋芽茎段能直接诱导出大量丛生芽,随后将不定芽转入MS添加IBA和KT的培养基中,可生成不定根,完成快速繁殖技术体系。结果表明:活血丹愈伤组织诱导的最佳培养基为MS+2,4-D(1.5mg/L)+BA(1.0mg/L),诱导率高达91.38%。丛生芽诱导的适宜培养基为MS+NAA(0.1mg/L)+BA(1·0mg/L),在此培养基上,出芽率达100%,芽增殖系数接近于10,有利于生物量的积累。而根的诱导则在MS+IBA(1.0mg/L)+KT(1.0mg/L)培养基上进行最好,此基础上能诱导出健康、粗壮的根。试管苗炼苗后移栽,成活率达100%。

Tissue culture and rapid propagation of Glechoma longituba were systematically studied in order to explore suitable medium and culture conditions. The leaf as explants could induce the callus on the MS with 2,4-D and BA. And it was studied with the different conditions of subculture and analyzed the cause of browning phenomenon. The stem tips and the stems with auxiliary-bud could induce cluster bud, then cluster buds were replanted on the MS medium with IBA and KT,indefinite-root would be induced and the system of fast propagation would be set up. The results showed that：The best medium for the induction of tissue culture was MS＋2,4-D（1.5 mg/L）＋BA （1.0 mg/ L）,the inducing rate could reach 91.38%,and the optimum medium for cluster bud was MS＋NAA （0. 1 mg/L）＋ BA （1.0 mg/L）,the rate of sprout could be up to 100%. Propagating coefficient might be close to 10,it was propitious to the accumulation of biomass. And the optimum medium for the induction of roots was MS＋IBA （1.0 mg/ L） ＋KT （1.0 mg/L） ;it could induce healthy roots. The survival rate of plantlets transplanted in soil could be up to 100% after nursling plantlets.