多位点DNA指纹技术在保加利亚普通田鼠中的应用探讨

Studies on applicability of multilocus DNA fingerprinting in Bulgarian common vole (Microtus arvalis)

In the paper, DNA fingerprinting patterns of common vole (Microtus arvalis) were detected with HindIII, HinfI and microsatellite probe (GTG) 5 .The results showed that legible DNA fingerprints were not produced with HindIII and probe (GTG) 5, however HinfI and probe (GTG)5 produced abundantly informative DNA fingerprints. DNA fingerprints were individual-specific and represented higher polymorphism in number, intensity and distribution bands among individuals. The band-sharing rates (BSR) between individuals were determined by the DNA fingerprints. The average band numbers and average band-sharing rates in fragment length region (FLR) of >2.3 kb were 29.625±5.012 and 0.388±0.083 respectively. Correlative fingerprint-data with the population were calculated from average BSR. In this study, a technical basis is established for application of multilocus DNA fingerprinting in common vole.

Abstract： In the paper, DNA fingerprinting patterns of common vole （ Microtus arvalis ） were detected with HindlII, Hinfl and microsatellite probe （GTG）5. The results showed that legible DNA fingerprints were not produced with HindlII and probe （ GTG） 5, however Hinfl and probe （ GTG ） 5 produced abundantly informative DNA fingerprints. DNA fingerprints were individual-specific and represented higher polymorphism in number, intensity and distribution bands among individuals. The band-sharing rates （ BSR） between individuals were determined by the DNA fingerprints. The average band numbers and average band-sharing rates in fragment length region （ ELR） of 〉2. 3 kb were 29. 625 ±5. 012 and 0. 388 ±0. 083 respectively. Correlative fingerprint-data with the population were calculated from average BSR. In this study, a technical basis is established for application of multilocus DNA fingerprinting in common vole.