D-氨基半乳糖/内毒素诱导的急性肝衰竭与Th1/Th2失衡

Acute Hepatic Failure Induced by D-glactosamine/endotoxin and Th1/Th2 Imbalance

目的:研究Th1/Th2失衡与D-氨基半乳糖/内毒素所致大鼠急性肝衰竭的关系。方法:30只Wistar大鼠腹腔内注射D-氨基半乳糖/内毒素诱导大鼠急性肝衰竭模型(AHF组),另取30只腹腔内注射等量生理盐水为正常对照(N组)。两组动物于建模后3h、6h、12h、24h、48h、72h各取5只动物检测其血清丙氨酸转氨酶(ALT)与肝组织的病理形态学变化;ELISA检测不同时间点动物血清中Th1/Th2型细胞因子(IFN-γ/IL-4)水平的变化。结果:AHF组肝组织显示炎细胞浸润和明显坏死的急性肝衰竭特征,且各时间点血清ALT值均明显高于N组(P<0.05);AHF组与N组相比血清内Th1型细胞因子(IFN-γ)的增高在3h(P<0.01)、6h(P<0.01);Th2型细胞因子(IL-4)水平在各时间点无明显变化(P>0.05)。结论:在D-氨基半乳糖/内毒素所致大鼠急性肝衰竭模型中,存在Th1/Th2失衡,机体内发生了促炎反应,对此急性肝衰竭早期损伤起重要作用。

Objective：To research the relation of Th 1/Th2 imbalance and acute hepatic failure induced by D -glactosamine/endotoxin. Methods： Wistar rats with acute hepatic failure （AHF group, n = 30） were induced by intraperitoneal injection of D- glactosamine/endotoxin, normal controls （N group, n = 30） were injected with saline solution.sera Alanine Transaminase（ALT） were determined and liver pathological morphology were observed at 3 h, 6 h, 12 h, 24 h,48 h and 72 h following intraper/toneal injection, 5 rats per time point; Simultane- ously,sera Thl/Th2 cytokines （IFN- γ/IL- 4） were examined by ELISA. Results： Infiltration of inflammatory cells and necrosis of hepatic cells were marked in AHF group, ALT increased significantly in AHF group than in N group （P 〈 0.05） at all time points; Compared with N group, levels of sera Th 1 cytokine （IFN - γ） were obviously higher at 3 h（ P 〈 0.01 ） and 6 h（ P 〈 0.01 ） ; Th2 cytokine （IL - 4） weren＇t obviously higher at all time points（ P 〉 0.05）. Conclusion：Th1/Th2 imbalance was found in the acute hepatic failure induced by D- glactosamine/endotoxin, so pro - inflammatory response play important role in the AHF models at early phase.