川芎嗪联合左旋精氨酸对缺血—再灌注损伤兔心肌线粒体功能的影响(英文)

Effect of Ligustrazini and L-arginine on Function of Mitochondria in Myocardial Cell in the Reperfusion Injury after Myocardial Ischemia in Rabbits

目的探讨川芎嗪联用左旋精氨酸对心肌缺血-再灌注损伤时心肌细胞线粒体功能的影响。方法选用日本大耳白兔50只,随机分为正常对照组(A组)、心肌缺血-再灌注组(B组)、心肌缺血—再灌注+川芎嗪治疗组(C组)、心肌缺血—再灌注+左旋精氨酸治疗组(D组)和心肌缺血—再灌注+川芎嗪+左旋精氨酸治疗组(E组)。观察心肌线粒体呼吸功能、Ca2+浓度、丙二醛浓度、超氧化物歧化酶活性和心肌组织三磷酸腺苷(ATP)和能荷的变化。结果与A组比较,B组线粒体呼吸控制率、Ⅲ态呼吸速率和超氧化物歧化酶明显降低,Ⅳ态呼吸速率、Ca2+浓度和丙二醛显著升高,心肌组织ATP和能荷明显降低。与B组比较,C组、D组和E组线粒体呼吸控制率、Ⅲ态呼吸速率和超氧化物歧化酶明显升高,Ⅳ态呼吸速率、Ca2+浓度、丙二醛显著降低,心肌组织ATP和能荷明显增高;且与A组比较,E组上述指标均无明显差异。结论川芎嗪联用左旋精氨酸可通过降低氧自由基水平和减轻钙超载,而改善缺血—再灌注损伤心肌的线粒体功能。

Aim To study the effect of ligustrazimi （LGT） and L-arginine （L-Arg） on function of mitochondria in myocardial cell during myocardial ischemia-reperfusion injury （MIRI）. Methods 50 animals were randomly divided into five groups （ n = 10 in each） ： control group（A）, MIR group （B）, MIB ＋ LGT group（C）, MIR＋L-Arg group（D）, MIR＋LGT＋L-Arggroup（E）. The mitochondrial respiratory function, Ca^2＋ concentra- tion（ [ Ca^2＋ ]m）, malondialdehyde （MDA） content and supemxide dismutase （SOD） activity were determined. Meanwhile, the contents of ATP and EC in the myocardial tissue were measured, respectively. Results It was found that mitochondrial respiratory control rate （RCR）,state 3 （ST3）, SOD, ATP and EC levels of myocardial tissue inC, D, Egroupwereldgher than those of B group , butstate4 （ST4）, [Ca^2＋] m, MDA were lower. However, between E and A group, all panuneters had no significant differences. LGT and L-Arg can improve function of mitochondria in myocardial ceils in the reperfusion injury after myocardial ischemia by decreasing oxygen free radical level and Ca^2＋ overload in the mitochondria.