碱性成纤维细胞生长因子对大鼠心脏微血管内皮细胞血管形成及血管内皮生长因子受体1的影响

Effect of Basic Fibroblast Growth Factor on Inducing Mouse Cardiac Microvascular Endothelial Cells to Form New Blood Vessels and Vascular Endothelial Growth Factor Receptor-1

目的探讨碱性成纤维细胞生长因子对诱导大鼠心脏微血管内皮细胞形成管腔样结构的影响,以及对血管内皮生长因子受体Flt-1的作用。方法分离、培养大鼠心脏微血管内皮细胞。应用Matrigel检测不同浓度碱性成纤维细胞生长因子作用后心脏微血管内皮细胞形成管腔的情况。逆转录聚合酶链反应检测各组血管内皮细胞Flt-1mRNA的表达量。结果应用Matrigel可诱导心脏微血管内皮细胞管腔样结构形成,随着碱性成纤维细胞生长因子浓度升高(0、51、0和20μg/L),管腔样结构形成的数量逐渐增多,当碱性成纤维细胞生长因子浓度达到40μg/L时管腔样结构的数量有所减少(P<0.05)。Flt-1 mRNA的表达量随碱性成纤维细胞生长因子作用浓度的升高(0、5、10和20μg/L)而逐渐增高,当碱性成纤维细胞生长因子浓度达到40μg/L时其mRNA水平有所降低(P<0.05)。结论应用Matrigel可在体外诱导培养的大鼠心脏微血管内皮细胞形成管腔样结构。碱性成纤维细胞生长因子对大鼠心脏微血管内皮细胞形成管腔样结构有促进作用,并在一定范围内呈剂量依赖性关系。碱性成纤维细胞生长因子影响大鼠心脏微血管内皮细胞膜受体Flt-1的表达,在一定范围内亦呈剂量依赖性,且这种剂量依赖性同微血管内皮细胞管腔样结构形成的数量一定范围内呈现一致性。

Aim To study the effect of basic fibroblast growth factor （bFGF） on inducing cardiac mierovasonlar endotbelial cells （ CMEC ） to form new blood vessels and the role of vascular endothelial growth factor receptor-1 （ VEGFR- 1, Flt- 1 ）. Methods Mouse CMEC were isolated and cultured. CMEC was induced to develop tube structure by using Matrigel so as to detect the influence of bFGF on neovasuarization. Reverse transcription polymerase chain reaction （RT-PCR） assayed the ex- pression of Fit-1mRNA. Results CMEG can be induced to develop tube structure by Matrigel in vitro. The number of vessol gradually increased with the concentrations of bFGF step up （0, 5, 10, 20 μg/L） and decreased when bFGF was 40 μg/L （ P 〈 0.05 ）. The expression of Fit- 1 mRNA gradually increased with the concentrations of bFGF step up （ 0, 5, 10, 20 μg/L ） and decreased when bFGF was 40 μg/L （ P 〈 0.05）. Conclusion Mouse CMEC can be induced to develop tube structure by Matrigel in vitro. Mouse bFGF can promote CMEC to form new blood vessels and in certain rage show a dose-dependent manner. bFGF can enhance the expression of Fit-1 and in certain rage show a dose-dependent manner; this dose-dependent manner is similar to that of new blood vessels＇ quantity.