富含半胱氨酸蛋白61在球囊损伤大鼠腹主动脉中的表达变化

Cysteine-Rich 61 Expression in Rats Abdominal Artery After Balloon Injury

目的观察大鼠腹主动脉球囊损伤后富含半胱氨酸蛋白61表达的变化。方法对球囊损伤后不同时间段的大鼠腹主动脉标本采用组织形态学观察内膜增生,采用免疫组织化学、逆转录聚合酶链反应、免疫蛋白印记分析检测富含半胱氨酸蛋白61的表达。结果球囊损伤后可见新生内膜在术后7天出现,术后14天明显增厚,术后28天内膜继续增生,形成的新生内膜厚度不均,较假手术组明显增厚(P<0.01)。球囊损伤后7天、14天采用免疫组织化学、逆转录聚合酶链反应、蛋白印记分析检测富含半胱氨酸蛋白61的表达均明显增强,与假手术组比较差异有显著性(P<0.01)。28天时与假手术组比较差异没有显著性(P>0.05)。结论球囊损伤后富含半胱氨酸蛋白61的表达明显增强,提示富含半胱氨酸蛋白61可能在球囊损伤后内膜增生过程中起重要作用。

Aim To investigate the change of Cysteine-rich 61 in rats abdominal artery injured by balloon. Methotis Forty-eight Wistar male rats were studied. Balloon injury was conducted in abdominal artery respecfively. Rats were killed at different time points and abdominal artery were collected in time. Intimal hyperplasia was studied by histological morphology method. The expression of tissue Cysteine-rich 61 was determined by immunoistochemieally, reverse transcription poly- merase chain reaction and western-blot. Results Intimal hyperplasia was present at 7th day after balloon injury, became more obvious at 14th day and in progression at 28th day. No change was observed in control group. The expression of Cysteine-rich 61 increased significantly at 7th day and 14th day after balloon injury, and it was significantly different between injury group and control group（P〈0.01}. The expression of Cysteine-rich 61 was not significantly different between injury group and control group at 28th day （P〉0.05）. Conclusions Cysteinc-rich 61 expressed stronger in injury group than that in control group, which indicated that Cysteine-rich 61 may play an important role in intimal hyperplasia caused by balloon injury.