生脉溶液对超氧阴离子自由基作用的实验研究

Study on the Effects of Shengmai Solution on Superoxide Anion Free Radical in Vitro.

目的体外测定并评价生脉溶液对超氧阴离子自由基（O2）的影响及作用。方法建立能够产生超氧阴离子的二甲基亚砜（DMSO）碱性体系，配制不同浓度生脉溶液、VC溶液为实验组及对照组，采用电子顺磁共振（EPR）方法，测定加入不同浓度溶液后体系中超氧阴离子信号的强度。结果加入浓度为20％、40％生脉溶液时，体系中超氧阴离子信号增强，且浓度为20％时与相同浓度对照组比较，增强显著（P〈0．01）；加入生脉溶液浓度增高至60％、80％及100％时，超氧阴离子信号强度逐渐减少并接近零，与相同浓度VC溶液对照组比较，减少显著（P〈0．01）。结论体外实验表明，较低浓度的生脉溶液对体系中超氧阴离子产生且有增强作用，而浓度较高时对其具有直接清除作用；体外实验说明生脉溶液对超氧阴离子自由基具有双向调节作用。

Objective Study on the effects of Shengmai solution on superoxide anion free radical in vitro. Methods The superoxide anion radical which produced in alkaline dimethyl sulfoxide（DMSO） system was directly measured by electron paramegnetic resonance（EPR）.The signal intensity of drug group and control group was calculated. Results Shengmai solution increased superoxide anion radical when its concentration was less than 40%,while decreased when its concentration was more than 60%.Comparing with the control group,the increasing or decreasing of signal intensity had statistical significance when the concentration of Shengmai solution was 20%,60%,80% or 100%.Conclusion Shengmai solution has dual effects on superoxide anion radical in vitro.