高效液相色谱法测定醋酸曲安奈德注射液中醋酸曲安奈德含量

Assaying of triamcinolone acetonide acetate on triamcinolone acetonide acetate injection by HPLC

[目的]建立醋酸曲安奈德注射液中醋酸曲安奈德含量测定的方法.[方法]采用高效液相色谱法,流动相为甲醇∶水∶乙醚(62∶38∶2),流动速度为1.0mL/min,检测波长为240nm,测定醋酸曲安奈德注射液中醋酸曲安奈德含量.[结果]醋酸曲安奈德的线性范围为4.1～185.4mg/L(r=0.9996),回收率为100.8%,RSD为1.26%(n=9).[结论]采用高效液相色谱法测定醋酸曲安奈德注射液中醋酸曲安奈德含量的方法具有简便、准确、重现性好等优点.

OBJECTIVE To establish the method for determining the triamcinolone acetonide acetate on triamcinolone acetonide acetate injection. METHODS The contents of triamcinolone acetonide acetate on triamcinolone acetonide acetate injection was determined by HPLC, and the mobile phase was methanol-H2O- ethanol （62：38：2）, mobile velocity was 1.0 mL/min, and wavelength of detection was 240 nm. RESULTS The method was linear within the range of 4.1- 185.4 mg/L（ r = 0.999 6）, and the average recovery was 100.8%, and the RSD was 1.26% （n = 9）. CONCLUSION The HPLC is simple, accurate, and with good reproducibility for determining the triamcinolone acetonide acetate in triamcinolone acetonide acetate injection.