利用PCR方法检测转BT基因水稻

Detection of BT Transgenic Rice Gene by Polymerase Chain Reaction

应用PCR技术进行转基因水稻检测研究。本文以转基因水稻为材料,以聚合酶链式反应(PCR)方法为基础,选择适用于转基因食品安全性检验的核酸检测技术,针对转基因水稻中普遍存在的花椰菜花叶病毒(CaMV35S)启动子、胭脂碱合成酶(NOS)终止子、和转入苏云金杆菌(Bacillus thuringiensis,简写为Bt)基因水稻的Cry1Ac片段进行PCR检测,建立适合转BT基因水稻的检测方法。该方法简便快速、检测结果与标准及其他文献资料相符。

Polymerase Chain Reaction （PCR）, an important method for the identification of genomic modified foods （GMF）, was developed for detection of the BT transgenic rice gene. Using this method, 35S-promoter gene, NOS-terminator gene, and crylAc gene that commonly existed in transgenic rice were detected. Moreover, new methods based on PCR techniques were established for distinguishing Bt Transgenic Rice from the counterpart of non-GM products and the detection system and for correlating the marked genes and transgenic. The method was easy and fast, and the corresponding results were in accord with the standard or the reported data.