微卫星不稳定性在胃癌组织中的表达

Expressions of the microsatellite instability in gastric carcinoma

目的研究微卫星在胃癌发生中的作用及其与胃癌临床病理特征之间的关系。方法采用PCR、PCR一变性聚丙烯酰凝胶及银染法检测38例胃癌及相应的22例癌旁肠化生组织的MSI。结果胃癌MSI阳性率为39.5%(15/38),DIS 123和BAT 26的MSI阳性率略高于其它位点,癌旁肠化生组织MSI阳性率为27.3%(6/22),6例MSI阳性肠化生标本对应的胃癌组织均为MSI-H,MSI与胃癌的分化程度和发生部位有关。结论肠化生组织中MSI的进行积累可能在一部分胃癌的发生中起作用,MSI可能是胃癌的早期发展过程。

Objective To investigate the role of mierosatellite instability （MSI） in carcinogenesis of gastric eaneer, （GC） and its relationship with elinieopathologieal features of GC. Methods MSI was examined in 38 surgically reseeted GC specimens and 22 corresponding paratumor intestinal metaplasis （IM） samples using PCR,PCr-denatured polyaerylamide gel based methods .Results MSI was detected in 39.5 % （15/38） of GC and 27.3 % （6/22） of IM samples respectively. All the corresponding GC tissues of MSI ^＋ IM samples were MSI-H. there is a correlation between MSI and differentiation, location of GC. Conclusion The progressive accumulation of MSI in areas of IM way be role of gastric carcinogenesis. MSI may be an initial stage of gastric carcinogenesis.