兔骨髓基质细胞的体外分离培养及诱导成骨的实验研究

Experimental studies of osteogenic potentiality of rabbit bone marrow stromal cells in vitro

目的探讨兔骨髓基质细胞(BMSCs)的体外分离培养及定向诱导成骨的方法。方法选择10周龄新西兰兔,抽取其股骨大转子部骨髓,体外分离纯化得到BMSCs,再利用条件培养液将其向成骨方向定向诱导培养。采用碱性磷酸酶(ALP)和冯库萨(Von Kossa)染色方法,鉴定其成骨性能。结果BMSCs在培养皿中贴壁生长、增殖,经ALP和Von Kossa染色证实其有成骨潜能。结论兔BMSCs的体外培养增殖能力强,能诱导分化为成骨细胞,可以作为骨组织工程的种子细胞。

Objective To explore the optimal system of ostengenic potential of bone marrow stremal cells （BMSCs）from rabbit？ s marrow.Methods New Zealand rabbits 10 weeks of age were selected to extract bone marrow solution at greater trochanter of femur.BMSCs were cultivated by differentiation culture medium. Von Kossa staining and alkaline phosphatase （ALP） activity test were employed to assess BMSCs osteoblastic differentiation and the generation of calcified extracellular matrix .Results BMSCs were cultivated, differentiated and proliferated well in the differentiation culture medium with active function, which were confirmed by Von Kossa staining and ALP activity test. Conclusion BMSCs of rabbits cultured can proliferate well and can be induced to differentiate into osteoblasts in vitro. They can be used as seed cells for bone tissue engineering.