摘要
目的通过与维生素E的对比实验,进一步研究虾青素的抗氧化能力。方法分别采用硫氰酸铁法(FTC)、邻二氮菲-Fe2+氧化法以及DPPH·自由基测定法,研究虾青素和维生素E在抑制亚油酸的自氧化、清除羟自由基和DPPH·自由基的能力。结果虾青素能有效地抑制亚油酸的自氧化性,抑制能力明显高于维生素E;同时对羟自由基的清除率也高于维生素E,且在浓度为50μg/ml时,清除率可达95.98%;清除DPPH·自由基方面虽然略低于维生素E,但同样表现出较高的清除率,当浓度为80μg/ml时,清除率为97.0%。结论虾青素具有明显的抗氧化活性。
Objective: To investigate the anti-oxidation activity of astaxanthin. Method: Free radical scavenging properties were investigated in modified chemical system. The antioxidation activity of astaxanthin and vitamin E for linoleic acid autooxidation, hydroxy radical and DPPH radical was detected by spectrophotometry. Results: Astaxanthin could inhibit autooxidation of linoleic acid effectively and its inhibition and elimination rate to hydroxy radical was higher than vitamin E. The elimination rate was 95.98% at the concentration of 50μg/ml astaxanthin. Although the effect of astaxanthin on eliminating DPPH radical was slightly lower than vitamin E, but the elimination rate still high to 97.0% at concentration of 80 μg/ml. Conclusion: Astaxanthin has obvious anti-oxidation activity.
出处
《营养学报》
CAS
CSCD
北大核心
2007年第2期163-165,169,共4页
Acta Nutrimenta Sinica
基金
国家科技攻关计划项目(No.2001BA804A21)
关键词
虾青素
维生素E
抗氧化
astaxanthin
vitamin E
anti-oxidation