摘要
用RAPD方法,对来自韩国、美国和中国的26个栗疫病菌菌株进行了遗传变异分析。使用筛选的12个随机引物,共扩增了115个0.19～3.1 kb大小的扩增片段,其中多态性片段占61.7%。聚类分析结果,相似系数为0.92时,26个菌株分为两大组,一组由21个菌株组成,包括大部分韩国菌株和美国菌株;另一组包括部分韩国菌株和中国菌株。表明美国菌株和大部分韩国菌株的遗传相似性很高,部分韩国菌株有较大的变异,而中国菌株则表现出了遗传上的远缘关系。
Genetic variation of 26 strains of Cryphonectria parasitica from Korea, USA and China was investigated using random amplified polymorphic DNA （RAPD） of genomic DNA. 115 RAPD bands were amplified by polymerase chain reaction with 12 random primers, and 61.7% of which were polymorphic, the size of amplified RAPD bands ranged from 0.19 to 3.1 kb. The lineage of 26 strains based on cluster analysis of RAPD bands, could be classified as 1 big group and the big group included 17 strains of Korea and 4 strains of USA. From this result, it was showed that most strains of Korea were similar to each other, and also similar to strains of USA, and the genetic relationship with strain of China was very low.
出处
《林业科学研究》
CSCD
北大核心
2007年第2期283-286,共4页
Forest Research
基金
韩国科学财团(KOSEF)Brain Pool Program的资助
关键词
栗疫病
寄生隐丛赤壳
栗树
遗传变异
chestnut blight
Cryphonectria parasitica
Castanea spp.
genetic variation
