摘要
The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes.It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%,whereas strains A4,MSU-2 and MT232 had little difference in hairy root occurrence.Infect conditions had great influence on hairy root inducement,and the better ones are: acetosyringone concentration,30 mg·L-1;infection time,15 minutes;and culturing conditions of strains,temperature 28 ℃,28 hours shaking culture under dark environment with shaking.Pre-experiment on tissue culture system of peony was conducted,and got better medium composition as: WP+6-BA 0.5 mg·L-1+ IBA 0.1 mg·L-1+GA30.5 mg·L-1for base buds,and MS + 6-BA 2.0 mg·L-1+IBA 0.1 mg·L-1+GA3 0.5 mg·L-1for stem segments.
The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes. It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%, whereas strains A4, MSU-2 and MT232 had little difference in hairy root occurrence. Infect conditions had great influence on hairy root inducement, and the better ones are： acetosyringone concentration, 30 mg· L^-1; infection time, 15 minutes; and culturing conditions of strains, temperature 28 ℃, 28 hours shaking culture under dark environment with shaking. Pre-experiment on tissue culture system of peony was conducted, and got better medium composition as ： WP ＋ 6-BA 0.5 mg·L^-1＋ IBA0.1 mg·L^-1＋GA30.5 mg· L^-1 for base buds, and MS ＋ 6-BA2.0mg· L^-1＋IBA0.1 mg· L^ -1 ＋ GA3 0.5 mg · L ^-1for stem segments.
出处
《林业科学研究》
CSCD
北大核心
2007年第2期292-295,共4页
Forest Research
基金
国家林业局948项目(2004-4-12)的部分研究内容
关键词
发根农杆菌
牡丹
侵染
组织培养
毛状根
Agrobactrium rhizogenes
peony
infect
tissue culture
hairy root
