玉米抗寒基因的克隆与表达研究

Isolation and Expression of the Genes Involved in Tolerance to Low Temperature in Maize

用DDRT-PCR技术研究玉米自交系承18在常温和不同低温处理下基因表达的变化,分离了10条差异表达的cDNA。序列分析结果表明:其中部分与前人克隆的抗逆cDNA同源,部分与信号传导相关基因同源,部分与功能未知的cDNA同源。用Northern杂交方法对差异片段MCI16做了进一步鉴定,同时还对玉米Cat3、拟南芥CBF1和FAD3基因在不同低温处理的玉米幼苗中的表达特性进行了研究,结果表明:MCI16、Cat3等基因受低温的诱导表达,对提高玉米的抗寒力起积极作用。

This article was the study of differential gene expression in maize inbred Cheng18 treated with different low temperature by mRNA differential display technique. Total 10 differentially expressed cDNA fragments were isolated in the study. The results of sequence analysis showed that the differentially expressed cDNA fragments were homologous with cloned cDNA fragments of adversity resistant genes, signal transduction related genes and partial unknown function cDNA fragments. The changes in gene expression of MCI16（chilling induced cDNA fragment of maize） was further confirmed by the northem hybridization analysis. At the same time the northern hybridization analysis of Cat3 in maize, CBF1 and FAD3 in Arabidopsis with maize materials treated by different temperature were made. The results showed that MCI16 and Cat3 were chilling resistant related genes, the expression of them were enhanced under the conditions of chilling acclimation and chilling stress. They played positive roles in chilling resistant.