摘要
[目的]建立酱油中的氯丙醇的GC—MS法测定方法。[方法]采用3-氯-1，2-丙二醇（3-MCPD）的氘代同位素d5—3-氯-1，2-丙二醇（d5—3-MCPD）作内标，提取物经七氟丁酰基咪唑衍生后，用选择离子监测的毛细管柱气相色谱顺谱联用法测定3-氯-1，2-丙二醇的含量。[结果]方法灵敏度高，干扰少；相对标准偏差为1．9％～4．9％；回收率为89．6％～95．8％。[结论]方法准确可靠、干扰少，适宜酱油中氯丙醇的测定。
[Objective]A new method of determining the chloropropanols in soy using gas chromatography- mass spectrometry （GC-MS） was established. [Methods]Using d5-3-MCPD as deuterated internal standard,the Heptafluorobutyrylimidazole（HFBI） was added to the sample to derivatise chloropropanols,then the derivatised chloropropanols were analysed by GC-MS. [Results]The method has high sensitiving,the relative standard deviation was 1.9 %-4.90%. The recovery rate of sample was 89.6 %-95.8 %. [Conclusion]This is a accurate and reliable method to determine the chloropropanols in soy.
出处
《预防医学论坛》
2007年第4期337-338,共2页
Preventive Medicine Tribune
