乐东拟单性木兰组培再生系统的建立

Establishment of Highly Efficient Regeneration System of Parakmeria lotungensis. Chun et C. Tsoong Law

以成年树嫩枝为外植体建立了乐东拟单性木兰的组培再生系统。试验筛选MS为基本培养基，较好增殖培养基为：MS＋6BA0．2mg／L＋IBA0．05mg／L；光照强度控制在1000lx左右；继代周期以4周为宜，继代8次以后可进入快速、稳定增殖阶段，增殖系数可达3．5以上。1．5—2cm不定芽放入MS＋IBA1—3mg／L的根诱导培养基中培养15d后，转入不加任何激素的1／2MS根形成培养基中，12d左右可出根。

For the first time, the regeneration system of tissue culture of Parakmeria lotungensis（ Chun et C. Tsoong ）Law was established by utilizing its twigs from the mature tree as explants. MS was more effective than other materials as basic medium in the whole experiment. The best medium of proliferation was MS ＋ 6 - BA 0.2 mg/L ＋ IBA 0.05 mg/L, illumination intensity 1 000 lx, subcultures interval was 4 weeks. After sub cultured for 8 times, the proliferation was fast and stable,multiplication rate was 3.5. The buds 1.5 - 2 cm long w.ere planted into the root- induced medium （MS ＋ IBA 1 - 3.0 mg/L） , after 15 d under darkness, the buds were transferred into the root - formation medium （ 1/2 MS） without any hormone, rooting started at about 12 days.