高效液相色谱法测定姜赤凝胶中芍药苷含量

Determination of the Content of Paeoniflorin in Jiangchi Gel by HPLC

目的 建立测定姜赤凝胶剂中芍药苷含量的高效液相色谱法。方法 使用日本岛津LC-90A液相色谱仪，LC-10AD泵，SPD-10A紫外检测器，CLC-ODS色谱柱（5μm，150mm×60mm）；流动相：甲醇-水（45：55）；流速：1mL·min^-1，柱温32℃，检测波长：230nm。结果 该方法测定芍药苷在0．08-0．80μg·mL^-1范围内线性关系良好（r=0．9996）。芍药苷的平均回收率为100．61％，测定结果RSD为0．89％（n=6）。结论 该测定方法简单、精密度高、重现性好，是测定姜赤凝胶剂中芍药苷含量的可行方法。

Objective To establish a method for determinating paeoniflorin in jiangchi gel by HPLC. Methods The chromatographic conditions were as follows： CLC-ODS column（5μm, 150 mm×60 mm）used as the stationary phase, and methanol-water （45 ： 55） as mobile phase with a flow rate of 1.0 mL · min^-1 ,and the detecting wavelength 230 nm. Results A good linearity was obtained within the range of 0.08 ～ 0.80 μg·mL^-1 of paeoniflorin （r = 0.999 6）. The average recovery rate of paeoniflorin was 100.61% （ n = 6, RSD = 0.89% ）. Conclusion This method is simple, rapid and accurate which can be used to determine paeoniflorin contents in jiangchi gel.