高效液相色谱法测定郁金银屑片中盐酸小檗碱的含量

HPLC Determination of Berberine Hydrochloride Content in Yujinyinxie Pill

目的测定郁金银屑片中盐酸小檗碱的含量。方法HPLC,用十八烷基硅烷键合硅胶为填充剂（250 mm×4.6 mm）;流动相：乙腈-0.1%磷酸溶液（50∶50）（每100 mL加十二烷基磺酸钠0.1 g）;检测波长：265 nm;流量：1 mL.min^-1;进样量：10μL。结果盐酸小檗碱在0.0256-0.179μg之间与峰面积呈良好的线性关系,相关系数r=1,回收率为99.76%,RSD=0.80%。结论该法灵敏、简便、准确,可作为本品的定量分析方法。

OBJECTIVE To determine the Berberine hydrochloride content in Yujinyinxie Pill. METHODS The HPLC C18 column（250 mm × 4.6 mm）. RESULTS The linearity was obtained over the range of 0. 0256 - 0. 179 pg（ r = 1 ）. The average recovery was 98.59% ,with RSD = 0.80% . CONCLUSION This method is sensitive, simple, specific and accurate for the control of Yujinyinxie PiU.