外源DNA导入高梁的叶部病害变异及其分子验证

Leaf Diseases Resistance Variation and Its RAPD Check of Sorghum Transferred Progenies by Exogenous DNA Introduction

通过花粉管通道,将抗叶部病害能力强的热带高粱DNA导入后,有27个导入后代的抗病性有所提高,占导入后代的9．1％。其中有5个导入后代的抗病性提高了2级,3个导入后代的抗病性提高了3级,显著地提高了受体的抗病性。表明供体DNA片段已通过花粉管通道进入受体,并对受体基因的表达产生影响。此项技术实现了地理远缘的品种间的遗传物质转移,丰富了遗传类型。

Exogenous DNA with leaf diseases resistance of tropical sorghum was introduced through pollen tube pathway. 27 transferred progenies＂ diseases resistance were increased, accounting for 9. 1 %,in which 5 transferred progenies＂ diseases resistance increased 2 grade and 3 transferred progenies＂ diseases resistance increased 3 grade. The disease resistance of receptor was increased significantly. This indicated that,donor DNA fragment had inserted into receptor through pollen tube, and affected the expression of genes in receptors. This technique realized the transference of inheritable matter between varieties of distant geography, and made the inheritable types abundant.