HPLC法测定清热润通胶囊中大黄素及大黄酚的含量

Determination of Emodin and Chrysophanol in Qingre Runtong Capsule by HPLC

目的:测定清热润通胶囊中大黄素及大黄酚的含量。方法:采用高效液相法,色谱柱为YWGC18柱(250mm×4.6mm,5μm);甲醇-0.1%磷酸溶液(85∶15)为流动相;柱温25℃,检测波长为254nm,流速为1.0mL/min。结果:大黄素的线性范围为22.9μg～183.2μg,大黄酚的线性范围为20.1μg～160.8μg;平均加样回收率,大黄素为97.92%,RSD=1.75%(n=5),大黄酚为97.62%,RSD=1.18%(n=5)。结论:该方法简便、快速、准确,可用于清热润通胶囊的质量控制。

Objective：To establish a HPLC method for determination the contents of emodin and chrysophanol in Qingre Runtong capsule. Methods：HPLC was performed on YWG-C18 （250 mm×4.6 mm, 10μm）column with methanol-0.1% phosphoric acid（85：15）as mobile phase at flow rate of 1.0 mL/min. The column temperature was 25℃ and the detection wavelength was 254 nm. Results：The liner ranges of emodin and chrysophanol were 22.9 μg-183.2 μg and 20.1 μg- 160.8 μg, The average recovery rate of emodin was 97.92% （RSD=1.75% ,n=5）and that of chrysophanol was 97.62% （RSD=1.18%, n=5）. Conclusion：This method is simple, quick, accurate, which can be used for the quality control of Qingre Runtong capsule.