针刺对实验性快速性心律失常大鼠心肌GimRNA表达的影响

Study on Acupuncture Experiment Tachyarrhythmia Rat GimRNA Expression in Myocard

目的:探讨针刺内关穴调节快速性心律失常的效应机理。方法:电针乌头碱所致的快速性心律失常模型大鼠双侧"内关"穴,观察针刺前后心律(率)变化;用半定量逆转录聚合酶链反应(RT-PCR)方法测定心肌中抑制性G蛋白(Gi)mRNA的相对含量。结果:针刺治疗组与模型组相比有显著变化(P<0.05)。结论:快速性心律失常可能和心肌中G蛋白基因表达障碍有关;G蛋白在针刺内关穴对心律失常的调节中有重要作用。

Objective： To explore mechanism of acupuncture Neiguan point to tachyarrhythmia. Methods： Tachyarrhythmia was induced with aconitine, and acupuncture two - side ＂Neiguan＂ by electro - acupuncture later, Observe the change cardiac rhythm and rate;The expression of GimRNA in myocard was detected by using reverse transcription polymerase chain reaction（ RT- PCR）. Results： Acupuncture treatment group has signifcent change compared with model group（ P 〈 0.05 ）. Conclusions： tachyarrhythmia probably related with G protein genetic in myoeard expression disordering, which was very important to regulate arrhythmia by acupuncture Neiguan point.