苦瓜蛋白对小鼠移植性肝癌H_(22)肿瘤的抑制作用

The Anti-Tumor Effects of Momordin from Momordica Charantia Seeds on Murine Transplanted Tumor H_(22)

目的:探讨苦瓜蛋白对荷瘤小鼠肿瘤生长和免疫器官的影响。方法:将清洁级ICR雄性小鼠按体重随机分成4组,建立肝癌H22皮下移植瘤模型,分为生理盐水对照组(con)、高剂量组(t3)、中剂量组(t2)和低剂量组(t1)。在普通饲料饲喂的基础上采用腹腔注射给药法分别予空白对照组给以生理盐水;高、中、低剂量组分别给以不同剂量苦瓜蛋白。观察其对肿瘤生长的影响;并观察其对肝、脾组织脏器指数的影响。结果:苦瓜蛋白干预组与对照组相比,低、中、高组抑瘤率分别为52.05%、66.89%及85.81%;与对照组比较,体重、瘤重等指标变化均具有统计学意义(P<0.05);肝、脾组织脏器指数变化等方面无统计学差异(P>0.05)。结论:苦瓜蛋白对在体肿瘤的生长具有显著抑制作用,对荷瘤小鼠的肝脾等免疫器官无明显抑制作用。

Objective： To explore the anti - tumor activity and effects of Momordin from Momordica Charantia Seeds on Murine Transplanted Tumor H22 and immune organ such as spleen and liver. Methods ： Mice of ICR species which had got the transplanted tumor H22 were divided randomly into four groups：the normal saline treated control group,low, middle and high dose Momordin treated mouse and labeled with t1 and t2 and t3 group,All of the mouse received the medicines such as normal saline and gradient dose of Momordin through peritoneal injection on the based of ordinary feeding.Anti - tumor activities, as well as the changes of animal weights and their immune organ such as liver and spleen index were also investigated. Results： Low, middle and high dose of Momordin treated group had a tumorostatic rate 52.05 % and 66.8% and 85.81% respectively, and meanwhile an evident inhibitory effect on body weigh was also seen （ P 〈 0.05 ）, but not on the liver and spleen index （ P 〉 0.05 ）. Conclusion： Momordin in a certain dosage show a marked inhibitory effect on the murine transplanted tumor H22, no harm to their immune organs such as spleen and liver.