消瘤汤干预大肠癌细胞凋亡相关基因bcl-2 bax表达的实验研究

The Experimental Study of Xiaoliutang's Influence on the Expression of the Genes bcl-2 and bax Concerned with the Apoptosis of Human Large Intestine Cancer Cell

目的:探讨消瘤汤促进大肠癌细胞株HT-29凋亡的作用机制。方法:采用MTT法测消瘤汤对大肠癌细胞株HT-29作用的有效剂量,运用流式细胞仪测定消瘤汤对人大肠癌细胞株HT-29凋亡相关基因bcl-2、bax表达的影响。结果:消瘤汤可促进大肠癌细胞株HT-29凋亡;能抑制凋亡相关基因bcl-2、bax的表达,但对凋亡抑制基因bcl-2的抑制更明显。结论:消瘤汤能促进肿瘤细胞凋亡,其作用机制可能是通过抑制凋亡相关基因表达实现的。

Objective： To study the influence of Traditional Chinese Compound Recipe Xiaoliutang decoction on the expression of bcl- 2, bax concerned with the apoptosis. Metbods： （1） The sensitivity of Xiaoliutang to the large intestine cancer was detected by MTT method. （2） The influence of Xiaoliutang decoction on the expression of bcl - 2 and bax was detected by FCM. Results： （ 1 ） Xiaoliutang has the function of restraining the multiplication of large intestine cancer HT - 29 （ P 〈 0.05 ） ; （2） The results of FCM indicates that Xiaoliutang has the function of promoting the apoptosis and depressing the expression of bcl - 2 and bax concerned with the apoptosis, especially on bcl - 2 （ P 〈 0. 05 ）. Conlusion： Xiaoliutang has the function of promoting the apoptosis, and the mechanisms of which is to depress the expression of bcl - 2 and bax.