丹星通络汤对脑缺血再灌注大鼠Caspase-3表达的影响

Influence of Danxing Tongluo Decoction on Nerve Cell Caspase-3 Expression in Rats with Cerebral Ischemia Reperfusion Injury

目的:观察丹星通络汤对大鼠脑缺血再灌注损伤神经细胞Caspase-3蛋白表达的影响,探讨其神经保护作用机制。方法:40只健康雄性Wistar大鼠,采用大脑中动脉线栓法建立局灶脑缺血再灌注大鼠模型,并随机将大鼠分为假手术组(Sham)、缺血再灌注组(I/R)、丹星通络汤大、小剂量组和尼莫地平组,每组8只。丹星通络汤组分别按15mL/kg(大剂量),7.5mL/kg(小剂量),尼莫地平组10mL/kg,术前连续灌胃给药7天,每天1次。假手术组和缺血再灌组分别灌以10mL/kg的生理盐水。大鼠局灶性脑缺血2h,然后进行再灌注。在再灌注24h断头取脑组织,采用免疫组织化学法检测脑组织中Caspase-3蛋白的表达,并与病理组织学改变进行对照。结果:丹星通络汤能明显降低大脑皮质及海马Caspase-3的表达,减轻神经细胞损伤。结论:丹星通络汤能减轻脑组织的缺血再灌注损伤,改善神经功能缺失,其作用机制可能与抑制Caspase-3蛋白表达有关。

Objective： It is to observe the influence of Danxing Tongluo decoction（DXTLD） on nerve cell Caspase-3 expression in rats with cerebral ischemia reperfusion injury and to study its nerve protection mechanism. Methods ： Forty healthy male Wistar rats were randomly divided into 5 groups： sham operation group, ischemia/reperfusion （I/R） group, DXTLD high dosage group, DXTLD low dosage group and Nimodipine（NIMO） group ,8 rats per group. DXTLD groups were given to DXTLD at dose of 15mL/kg,7.5mL/kg;NIMO at 10mg/kg,one time a day. Saline Were given to sham operation group and I/R group at 10mL/kg weight. After 7 - days of intragastried. The rats of all the groups except sham group were made into cerebral ischemia reperfusion injury models by thread embolism method. All the rats were killed and their brains were taken out after 2h ischemia and 24h reperfusion injury. The Caspase - 3 expression in brain tissue was measured with immunohistochemical method and was compared with the change of pathohistology. Results ： DX- TLD could obviously decrease the Caspase -3 expression in cerebral cortex and hippocampus and relieve nerve cell injury. Conclusion： DXTLD can protect brain tissue from cerebral ischemia reperfusion injury and improve the nerve function. The mechanism may be related with the decreasing of Caspase -3 expression.