HPLC测定四季感冒片中5-O-甲基维斯阿米醇苷含量

Determination of 5-O-Methylvisammioside in Siji Ganmao Tablet by HPLC

采用高效湘相色谱法测定四季感冒片中5-O-甲基维斯阿米醇苷含量。色谱柱：Diamonsil^TM（钻石）C18柱（5μm,200mm×4．6mm）；流动相：乙腈-水溶液（1：5）；流速：0．65mL／min。检测波长：290nm。5—O-甲基维斯阿米醇苷对照品进样量在5．05-35．35μg／mL范围内呈现良好线性关系，r=0．9997，平均回收率为101．1％。RSD为1．224％（n=6）。本方法简便，重现性好。

A HPLC method for the determination of 5 - O- Methylvisammioside in Siji Ganmao tablet was established. A Diamonsil^TM C18 column（5μm,200mm ×4.6mm） was used with acetonitrile-water（ 1：5 ） as mobile phase. The flow rate was 0.65mL/min. Content was detected at a wavelength of 290nm. The linear range was 5.05 - 35.35 μg/mL, r = 0.9997. The average recovery rate was 101.1% ,RSD = 1.224% （ n =6）.The method was simple,accurate and reproductive.