表达谷胱甘肽S转移酶-蜂毒素-^(88)精氨酸变体白细胞介素2工程菌的发酵条件研究

Study on the fermentation conditions of E.coli expressing GST-Melittin-^(88)ArgIL-2

目的研究重组蜂毒素-88精氨酸变体白细胞介素2(Melittin-88ArgIL-2)工程菌的发酵条件。方法采用摇瓶发酵,对含pGEX-4T-2-Melittin-88ArgIL-2重组质粒的工程菌进行诱导表达,研究诱导温度、诱导时间、诱导剂浓度、培养基的pH值等对谷胱甘肽S转移酶-Melittin-88ArgIL-2表达量的影响。同时研究培养温度对产物表达形式的影响。结果根据优化的条件,蛋白质的表达量占菌体总蛋白质的36%左右。结论确立了该重组melittin-88ArgIL-2工程菌的发酵条件。

Purpose To study the best fermentation conditions of E. coUi expressing GST-Melittin-^88 ArgIL-2. Methods The range of medium pH,induction temperature, induction time and revulsivum concentration and expression of the recombinant protein were analyzed and optimized with fermentation bottle. Results Under the established conditions, expression quantity of recombinant GST-Melittin-^88ArgIL-2 was about 36 % of total protein in the host. Conclusion The fermentation condition of E. colli expressing GST- Melittin-^88 ArgIL-2 was established.