浓香型白酒曲药中细菌组成及系统学分析

Phylogenetic Analysis of Bacterial Composition In Luzhou-flavor Liquor Quyao

利用免培养法(Culture independent approach)提取浓香型白酒曲药中细菌的总DNA,在分子水平上运用PCR扩增技术和16S rDNA序列同源性分析等方法测定曲药中细菌的16SrDNA基因近全序列,并建立系统发育树图。结果表明,组成该曲药中的细菌分属于Delftia、Dys-gonomonas、Bacteroidetes、proteobacterium、Nocardiopsis、Pseudomonas和Arthrobacter几大类群(phylum),表现出高度的细菌多样性。

In order to know the bacterial composition in Luzhou-flavor liquor Quyao, the techniques including culture independent approach, PCR technique and 16S rDNA sequence homology analysis etc. were used to measure 16S rDNA gene total order of bacteria in Quyao and the phylogenetic tree was further established. According to the sequences alignment and through the analysis of its comparability, the bacteria of Luzhou-flavor Quyao were consisted of Delfiia, Dysgonomonas, Bacteroidetes, proteobacterium, Nocardiopsis, Pseudomona and A rthrobacter, which indicated their bacterial diversity.