期刊文献+

酿酒酵母中的蔗糖转换酶基因(SUC2)研究进展 被引量:5

Research Progress on SUC2 Gene in Saccharomyces cerevisiae
下载PDF
导出
摘要 蔗糖转换酶基因(SUC2)编码蔗糖转换酶(Invertase),通过降解酵母细胞外的蔗糖成果糖和葡萄糖来提供酵母生长所需的碳源,在以蔗糖为主要碳源的培养条件下,蔗糖转换酶对于酵母生长必不可少,因此研究蔗糖转换酶基因序列、转录、调控等对酵母蔗糖代谢具有重要意义。蔗糖转换酶基因的表达受葡萄糖浓度、氧气和其在染色体上的位置等因素的影响。本文就葡萄糖浓度对SUC2表达的影响以及葡萄糖浓度对其表达调控的机制进行了综述,并展望了该基因及蔗糖转换酶在今后的应用和研究趋势。 SUC2 mainly encodes sucrose invertase. The invertase could hydrolyse sucrose into glucose and fructose which are carbon source for yeast growth. When sucrose is used as the main carbon source, invertase is necessary for the growth of yeast. Accordingly, it is of great significance for yeast glycometabolism to study the sequence, transcription and regulation of sucrose invertase. The expression of SUC2 is influenced by the concentration of glucose, oxygen and the position of SUC2 in the chromosome. In this paper, the effects and the regulation mechanism of glucose concentration on SUC2 expression were reviewed and the use of such gene and the invertase in the future and the research trend were predicted.
出处 《酿酒科技》 北大核心 2007年第5期85-88,共4页 Liquor-Making Science & Technology
关键词 酿酒酵母 蔗糖转换酶 蔗糖转换酶基因(SUC2) Saccharomyces cerevisiae invertase SUC2
  • 相关文献

参考文献33

  • 1Carlson M,Bostcin D.Two di.erentially regulated mRNAs with di.erent 5'ends encode secreted and intracellular forms of yeast invertase[J].Cell,1982,28:145-154.
  • 2Sarokin L,Carlson M.Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccha romyces cerevisiae[J].Mol Cell Biol,1984,(4):2750-2757.
  • 3Xiaoli Zhang,Zlmojie Xia,Binxia Zhao et al.Enhancement of production of clonext α-amylase by lactic acid feeding from recombinant Saccharomyces cerevisiae using a SUC2 promoter[J].Biotechnology Letters,2001,23:259-262.
  • 4Cha HJ.Kim M-H,Kim SH et al.Enhancement,by succinate addition,of the production of cloned glucoamylase from recom binant yeast using a SUC2 promoter[J].Process Biochem.1998.33:257-261.
  • 5Cha HJ,Yoo YJ.Determination of optimal glucose concentrations for glucoamylase production from plasmid-harboring and chromosome-integrated recombinant yeasts using a SUC2 promoter[J].Process Biochem.1996,31:499-506.
  • 6Lin KH,lijima S,Shimizu K et al.Control of gene expression from SUC2 promoter of Saccharomyces cerevisiae with the aid of glucose analyzer[J].Appl,Microbiol.Biotechnol,1989,32:313-316.
  • 7Jang JK,Pyun YR,Seo JM.Analysis of cloned SUC2 gene expression in continuous culture of recombinant Saccharomyces cerevisiae[J].Biotechnol,Bioeng,1990,36:960-964.
  • 8Sabire ozcan,Laura G,Vallier et al.Expression of the SUC2 gene of Saccharomyces cerevisiae is induced by low levels of glucose[J].Yeast,1997,13:127-137.
  • 9Vitolo M,Vairo MLR,Borzani W.Invertase activity of inact cells of Saccharomyces cerevisiae growing on sugarcane classes.Ⅱ.Unsteady-state ontinuous-culture tests[J].Biotech ioeng,1987,30:9-4.
  • 10Dodyk F,Rothestein A.Factors in uencing the appearance finvertase in Saccharomyces cerevisiae[J].Arch Biochem Bio-hys,1980,104:478-186.

共引文献2

同被引文献60

引证文献5

二级引证文献21

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部