HPLC法测定梅鹿辄和黑比诺葡萄中的花色素苷

Determination of Anthocyanin in Merlot Grape and in Pinot Noir Grape by HPLC

利用甲醇和超声波提取酿酒葡萄梅鹿辄和黑比诺中的花色素苷,利用高效液相色谱测定该葡萄中的花色素苷。在梅鹿辄葡萄果实中共检测到9种花色素苷,以二甲花翠素3-O-葡萄糖苷、二甲花翠素3-O-(6-O-乙酰)葡萄糖苷和二甲花翠素3-O-(6-O-对香豆酰)葡萄糖苷为主,这三者占总成分的79.69%,构成了梅鹿辄葡萄果实的主要呈色成分。在黑比诺果实中只检测到5种花色素苷,其中二甲花翠素3-O-葡萄糖苷和甲基花青素3-O-葡萄糖苷构成了黑比诺葡萄果实的主要呈色物质,占总成分的94.18%。

Anthocyanin in Merlot grape and in Pinot Noir grape was extracted by methanol and ultrasonic and then it was detected by HPLC. 9 kinds of anthocyanins were detected in Merlot grape mainly including Malvidin 3-O-Glu, Malvidin 3-0- （6-O-Acetyl）-Glu and Malvidin 3-0- （6-O-P-Coumaryl）-Glu which accounted for 76.69 % of the total anthocyanins and were the main coloration compositions of Merlot grape. Only 5 kinds of anthocyanins were detected in Pinot Noir grape mainly including Malvidin 3-O-Glu and Peonidin 3-O-Glu （the main coloration compositions） which accounted for 94.18 % of the total anthocyanins.