全脊髓匀浆建立豚鼠实验性自身免疫性脑脊髓炎模型

ESTABLISHMENT OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS MODEL ON GUINEA PIG

目的:用全脊髓匀浆(粗制髓鞘碱性蛋白)建立豚鼠实验性自身免疫性脑脊髓炎(EAE)模型。方法:Wistar大鼠全脊髓匀浆+完全福氏完全佐剂(CFA)免疫豚鼠,观察豚鼠的临床症状和中枢神经系统(CNS)的病理改变。结果:实验豚鼠均表现典型的EAE症状;光镜观察见CNS血管周围炎性细胞浸润和白质脱髓鞘改变。结论:全脊髓匀浆加完全福氏完全佐剂成功的诱导了豚鼠的EAE模型,具有模型稳定、发病率高、动物廉价易得的优点,是研究多发性硬化理想的动物模型。

Objective：To establish the experimental autoimmune encephalomyelitis （EAE） model on Guinea Pig with pan-spinal cord homogenate （coarse myelin basic protein,cMBP）.Methods：The Guinea Pig was immunized with Wistar rat pan-spinal cord homogenate ＋Freund＇s complete adjuvant（CFA）.The clinical symptoms and histopathologic changes of central nervous system were observed.Results：The Guinea Pigs in experimental group all demonstrated the typical symptoms of EAE with an incidence of 100%. The inflammatory cuff around small blood vessels, perivascular inflammatory cell infiltration and demyelination in white matter were observed through optical microscope.Conclusion：The EAE model of Guinea Pig was induced successfully ,the model was stable and with high incidence; besides the Guinea Pig was cheap and easy to get.Thus this model might be an ideal animal model to study multiple sclerosis.